Allelic variation and prevalence of serum opacity factor among the Streptococcus suis population, by D. Takamatsu, M. Osaki, P. Tharavichitkul, S. Takai and T. Sekizaki
Journal of Medical Microbiology vol. 57, part 4, pp. 488 - 494
Supplementary Table S1
Isolates and strains used in this study. [PDF file] (175 KB)
Supplementary Fig. S1
(a) Positions of primers used for preparation of the ofs probe. The PCR product was amplified from S. suis NIAH 11433 genomic DNA. (b) Genomic DNA Southern blot hybridization analysis of S. suis isolates with different ofs genotypes. Since there are no ClaI sites in any of the ofs genes represented in Fig. 2, genomic DNA was digested with ClaI. The ofs probe hybridized with only a single ClaI fragment in the isolates with types-1–3 ofs and MNCM51, while the probe hybridized with two ClaI fragments in the isolates with divided ofs loci, including strain 89/1591. In the ofs negative isolates, no signal was detected by the ofs probe. [PDF file] (506 KB)
