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J Med Microbiol 57 (2008), 980-985; DOI: 10.1099/jmm.0.47663-0
© 2008 Society for General Microbiology
ISSN 1473-5644

Presence of pathogenic Borrelia burgdorferi sensu lato in ticks and rodents in Zhejiang, south-east China

Chen-Yi Chu, Bao-Gui Jiang, Wei Liu, Qiu-Min Zhao, Xiao-Ming Wu, Pan-He Zhang, Lin Zhan, Hong Yang and Wu-Chun Cao

Department of Epidemiology, Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, PR China

Correspondence
Wu-Chun Cao
caowc{at}nic.bmi.ac.cn

Received 29 September 2007
Accepted 26 March 2008

A molecular epidemiological survey was conducted to investigate the presence of pathogenic Borrelia burgdorferi sensu lato (s.l.) species in the forest areas of Zhejiang province, south-east China. A total of 182 ticks of 6 species and 200 rodents of 8 species were collected and individually examined for the presence of B. burgdorferi s.l. DNA by nested PCR targeting the 5S–23S rRNA intergenic spacer. Forty-one ticks of four species, Haemaphysalis concinna, Haemaphysalis longicornis, Rhipicephalus microplus and Haemaphysalis warburconi, were infected with B. burgdorferi s.l., with an overall infection rate of 23 %. Sixteen rodents of four species, Nivivener confucianus, Nivivener coxingi, Apodemus sylvaticus and Rattus losea, were positive for B. burgdorferi s.l., with an overall prevalence of 8 %. MseI RFLP analysis and sequence analysis of the positive PCR products showed that Borrelia spirochaetes in specimens consisted of Borrelia garinii, Borrelia afzelii and Borrelia valaisiana-related group. Forty (98 %) of the B. burgdorferi s.l.-positive ticks were infected with B. garinii and one (2 %) was infected with B. afzelii. Twelve (75 %) of the positive rodents were infected with B. garinii and four (25 %) were infected with the Borrelia spirochaete belonging to B. valaisiana-related group.

Abbreviations: LD, Lyme disease.

The GenBank accession numbers for 5S–23S rRNA intergenic spacers reported in this study are EU160458 and EU160459.






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