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J Med Microbiol 57 (2008), 974-979; DOI: 10.1099/jmm.0.2008/001388-0
© 2008 Society for General Microbiology
ISSN 1473-5644

Potency of IMP-10 metallo-β-lactamase in hydrolysing various antipseudomonal β-lactams

Wei-Hua Zhao, Zhi-Qing Hu and Tadakatsu Shimamura

Department of Microbiology and Immunology, Showa University School of Medicine, Tokyo 142-8555, Japan

Correspondence
Wei-Hua Zhao
whzhao{at}med.showa-u.ac.jp

Received 19 February 2008
Accepted 31 March 2008

Limited β-lactams show antipseudomonal activity. The rapid spread of IMP-type metallo-β-lactamases (MBLs), which have a broad spectrum of substrates and a poor susceptibility to clinically available inhibitors, further restricts β-lactam use. In the present study, we evaluated the potency of IMP-10 MBL in hydrolysing antipseudomonal β-lactams currently available in the clinic. Crude IMP-10 MBL was prepared from two clinical isolates of Pseudomonas aeruginosa harbouring the blaIMP-10 gene. The sensitivity of β-lactams to hydrolysis by IMP-10 MBL was determined by comparing the MICs of 14 antipseudomonal β-lactams against a susceptible strain of P. aeruginosa in the presence and absence of IMP-10 MBL. Carbapenems (imipenem, meropenem and panipenem) and extended-spectrum cephems (ceftazidime, cefoperazone, cefsulodin and cefepime) were sensitive to the hydrolysing activity of IMP-10 MBL. By comparison, the fourth-generation cephem (cefpirome), the extended-spectrum penicillins (carbenicillin, ticarcillin, piperacillin and mezlocillin) and monobactams (aztreonam and carumonam) were relatively resistant to IMP-10 MBL. The sensitivity profile of antipseudomonal β-lactams to IMP-10 MBL generated in the present study provides a valuable reference for antibiotic selection by medical professionals.

Abbreviations: AZT, aztreonam; CAZ, ceftazidime; CBPC, carbenicillin; CFPM, cefepime; CFS, cefsulodin; CPR, cefpirome; CPZ, cefoperazone; CRMN, carumonam; IPM, imipenem; MBL, metallo-β-lactamase; MEPM, meropenem; MZPC, mezlocillin; PAPM, panipenem; PCase, penicillinase; PIPC, piperacillin; TIPC, ticarcillin.






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